Tuesday, April 11, 2006

Technical Hurdles

Technical Hurdles

Cloning is quite inefficient and usually there are over 600 to 1000 nuclear transfers before one is able to grow into a stem cell. This inefficiency is thought to be due to genetic imprinting in the cloned adult cell that interferes with the correct gene expression in the embryo. Even those animals that are successfully cloned are not as healthy as the original animal. For example, Dolly had arthritis and sign of premature aging. See methylation and epigenetic.
PublicationsCloning and Stem Cells, published by Mary Ann Liebert, Inc., publishes peer-reviewed research papers on the remarkable new opportunities in medicine, biology, and agriculture that arise from the demonstration of far greater than expected developmental plasticity in mammalian cells. Papers cover all aspects of cloning along with the culture and differentiation of stem cells from all stages of development from embryo to adult.

Cloning in fiction

Cloning in fiction

Cloning has been widely explored in science-fiction.
Brave New World (1932). An allegorical novel of social commentary. People do not reproduce sexually, but are cloned by embryo splitting, the way monozygotic (”identical”) twins actually occur, and the lower castes are chemically stunted. Describes the impact of an extrinsic noble Savage on a regulated and tranquilized World State. Cloning, David Shear (1972). Novel about a scientist who discovers he is a clone. His mind and body are taken over by the psyche of a genetic twin he never knew. Describes cloning by nuclear transfer, gene therapy, and growing replacement organs. Explicates the abortion debate and the criterion for death. Sleeper (1973). Woody Allen's comedic exploration of a dystopic future includes an attempt to resurrect an assassinated dictator by cloning him from the only surviving body part, namely his nose. The Boys from Brazil, novel (1976) and film adaptation (1978). Nazi Dr. Josef Mengele clones children from Adolf Hitler’s DNA in Brazil and sent to all over the northern world. Mengele tries to replicate Hitler's upbringing by killing the clone's 65 year old father. Parts: The Clonus Horror (1979). A science fiction film about an isolated community in a remote desert area, where clones are bred to serve as a source of replacement organs for the wealthy and powerful. Jurassic Park, novel (1990) and film (1993) by Michael Crichton. Vacation island populated with dinosaurs cloned using DNA from blood sucked by fossilized, prehistoric insects. Initially only females, they learn to reproduce. The enclosure fails and disaster ensues. Multiplicity (1996) Michael Keaton has himself cloned in order to get more accomplished. Gattaca (1997). Movie about the struggles of a man not genetically engineered, in a world where people produced by sexual union are considered unworthy of good professions. The letters in the name are A, T, G, and C, the elements of the genetic code in DNA. The 6th Day (2000). A film whose main themes are clones (e.g., the protagonist), and branching ethical cloning problems. The House of the Scorpion, Nancy farmer (2002). Young adult novel following the life of Matteo Alacran, a cloned young man who goes through a normal life, but soon finds out about his origin. Star Wars Episode II: Attack of the Clones (2002) and Star Wars Episode III: Revenge of the Sith (2005), two of the sequels in the George Lucas' film series. A species called Kaminoans use an accelerated cloning technique to create an army of over a million human soldiers that participate in a galaxy-wide conflict known as the Clone Wars. A Number, (2003) by Caryl Churchill . Play about a man who creates a clone to replace a son he's dissatisfied with, and what happens when they discover each other's existence 35 years later. Godsend (2004) Funeral home workers make an illegal clone The Island (2005). A film about the ethics of cloning. Follows the story of clones grown in a secret complex for the sole purpose of harvesting their organs for use as replacements in the originals, while they believe they are living independent, purposeful lives. Never Let Me Go (2005). by Kazuo Ishiguro details a world where clones are raised away from the public to provide organs for organ donation. Isolation (2005). A horror film about the effects of cattle cloning, where Large Offspring Syndrome goes haywire. Year Not Listed
Metal Gear Solid (year?) A man believed to be the best soldier of the 20th century, is cloned while comatose. The Clone (year?) by H.G. Wells, the historian and novelist who, along with the equally prescient Jules Verne, established the science fiction genre at the end of the 19th century. The Vorkosigan Saga (year?)by Lois McMaster Bujold has as a significant plot thread the cloning industry of the planet Jackson's Whole and the use of clones as spare bodies into which the brains of rich, elderly customers are transferred. In the episode of South Park titled An Elephant Makes Love to a Pig, Dr. Mephisto creates a clone of Stan, which turns out deformed, destructive, and retarded. The Third Twin (year?, by Ken Follett. Research scientist discovers that her boss has created dangerous, violent clones in a failed attempt to create "super-soldiers."